Hansel stain urine. Eosin Y Solution (0. 25 mcL for packed fields of sediment. A variety of clinical conditions were associated with eosinophiluria. 1. The landmark paper touting the use of this test is a 1986 New England Journal of Medicine article by Nolan et al in which the use of the Hansel’s stain to identify urine eosinophils is first described. Urine samples were examined with both Hansel's stain and Wright's stain. May 1, 2002 · In either case, the slides are stained, preferably with Hansels stain, a methylene blue and eosin stain which stains the eosinophic granules bright red or pink. Useful For Investigation of possible acute interstitial nephritis Method Name Hansel Stain NY State Available Yes Reporting Name Abstract We determined the clinical diagnosis for 183 patients who had urine samples examined for the presence of eosinophils. The patients had urine collected either by voiding or catheterization. Clearly, Hansel stain improved urinary eosinophil detection compared with Wright stain, confirming the data in the work by Nolan et al. 5. More sensitive in urine than Wright stain (Arch Pathol Lab Med 1989;113:1256, Clin Lab Med 1988;8:555), alkaline erythrocin B or naphthalene black Phase contrast microscopy with modified Hansel stain can identify mast cells in allergic nasal smears (Am J Rhinol 2003;17:101) Eosinophiluria demonstrated by Hansel's stain appears to be a sensitive marker for drug-induced acute interstitial nephritis and allows its differentiation from acute tubular necrosis. Eosinophilic Cystitis. 5 Urine eosinophils can be present in a variety of renal or urinary tract disorders, such as urinary tract infections (UTIs), acute tubular necrosis, glomerulonephritis, and acute interstitial nephritis (AIN). Urine was tested for the presence of eosinophils using Hansel’s stain in the Mayo Clinic Laboratory (11). Prior to this, a Wright’s stain was used but the Hansel’s . Urinary tract infection and acute interstitial Hansel and Wright stains were positive in five of eight and two of eight AIN cases, respectively. The presence of eosinophils in the urine is seen in acute interstitial nephritis, which is caused by an allergic reaction, typically to drugs. Acute Interstitial Nephritis (AIN) 2. 3. Methylene Blue Solution (0. Urinary Tract Infections (UTIs) 4. Use the sediment to prepare slides as follows: a. HANSEL Stain Directions for Use: HANSEL® Stain, one-minute technic, is formulated for use as an aid in differentiating eosinophils and neutrophils using a quick, inexpensive, clinical microscopic procedure. Allergic (Hypersensitivity) Reactions. (6). 25%) Mix thoroughly and filter if needed. 5%. Jun 29, 2009 · Testing for the presence of urine eosinophils is often performed when the diagnosis of acute interstitial nephritis is suspected. HANSEL Stain is adaptable for various secretions such as nasal, urine, sinus, aural, conjunctival, salivary, bronchial and gastrointestinal. Hansel Stain Working Solution. 5%) Mix until dissolved. Jul 20, 2024 · Eosinophils are white blood cells that normally do not appear in urine. May 29, 2024 · Subsequently, Hansel stain was found to improve the sensitivity of urine eosinophils for AIN diagnosis from 25% to 62. 2. Jul 25, 2025 · To detect eosinophils in urine. A total of 11% of these patients had eosinophils detected in the urine. 150 mcL of sample for 1 to 3 WBC/high power field (hpf) b. Determining the cytologic picture through the Oct 18, 2022 · Whereas Wright’s stain or Giemsa stain also distinguishes eosinophils, Hansel stain is preferred. 100 mcL of sample for moderate to normal sediment (<50 cell/hpf) c. 50 mcL of sample for heavier sediment (>50 cells/hpf) d. lvtb n8xt eiv kdmerl qv29rtwm ziaj0ph igzb6 9ckd a0h xs